Acetyl-11-keto-β-boswellic acid suppresses docetaxel-resistant prostate cancer cells in vitro and in vivo by blocking Akt and Stat3 signaling thus suppressing chemoresistant stem cell-like properties

摘要

Effect of AKBA on cell proliferation and apoptosis in docetaxel-resistant PCa cells. The in vitro effect of docetaxel on established human docetaxel-resistant PC3/Doc cells and parental PC3 cells. ***  b Cell viability in response to AKBA was determined by the MTT assay after 48 h of treatment in PC3 and PC3/Doc cells. The results are the means ± SD of three independent experiments. *  c The morphological changes after 48 h of treatment with 0, 10, 20, and 30 μM AKBA in PC3 and PC3/Doc cells. AKBA-induced cell death was detected by annexin V/PI staining and flow cytometric analysis. The expression of PARP in cells treated with AKBA at various concentrations was examined by western blotting. GAPDH served as the loading control. Cell viability in the absence or presence of the pan-caspase inhibitor Z-VAD-fmk. PC3/Doc cells were exposed to 10 μM Z-VAD-fmk for 2 h prior to 20 μM AKBA or vehicle treatment. The results are the means ± SD of three independent experiments. **