A solution for stripping antibodies from PVDF immunoblots for multiple reprobing

摘要

Available protocols for stripping antibodies from immunoblots involve the use of SDS or low pH buffers. SDS was shown to remove transferred proteins from membranes and low pH buffer shown to inefficiently strip off antibodies. A solution containing 6M guanidine hydrochloride, 0.2% non-denaturing detergent and a reducing agent can rapidly strip off tightly bound antibodies from aged PVDF immunoblots at room temperature, without removing significant amounts of transferred protein.