Proton Assisted Recoupling at High Spinning Frequencies

摘要

We demonstrate the successful application of ¹³C-¹³C proton assisted recoupling (PAR) on [U-¹³C,¹⁵N] N-ƒ-MLF-OH and [U-¹³C,¹⁵N] protein GB1 at high magic angle spinning (MAS) frequencies (ωr/2π=65 kHz). Specifically, by combining PAR mixing with low power heteronuclear decoupling (ω1H/2π~16 kHz) and high spinning frequencies, we obtain high resolution 2D spectra displaying long range ¹³C-¹³C contacts from which distances can be extracted. These experiments therefore demonstrate the possibility of performing high resolution structural studies in the limit of high spinning frequency and low power ¹H decoupling, a regime which optimizes resolution of protein samples and preserves their integrity.