Acquisition of chemiluminescent signals from immunoblots with a digital SLR camera

摘要

We found that certain mid-range consumer level digital SLR cameras using full-frame CMOS sensors outperform x-ray film in acquiring signals from immunoblots that use enhanced chemiluminescence for detection. These cameras exhibit a sensitivity comparable to x-ray film, yet provide a 3-fold increase in linear dynamic range and substantial cost saving over time, are more convenient to use, and eliminate the chemical waste associated with processing film.