Maintaining adequate nutrition not probiotic administration prevents growth stunting and maintains skeletal muscle protein synthesis rates in a piglet model of colitis

摘要

Malnutrition and cytokine-induced catabolism are pervasive in children with inflammatory bowel diseases (IBD), yet the benefits of aggressive nutrition support or of probiotics on nutrient and functional deficiencies and growth remain unclear. Piglets with dextran sulfate (DS)-induced colitis consuming a 50% macronutrient restricted diet (C-MR) were compared with those receiving probiotics (C-MRP) or adequate nutrition (C-WN) and with healthy well-nourished controls (REF). C-WN vs. REF had reduced growth (−34% chest circumference and −22% snout-to-rump length) and a tendency toward lesser weight gain, but showed no effects on skeletal muscle protein muscle fractional synthesis rate (FSR) or initiation of translation via the mTOR pathway. Compared to C-WN, the C-MR and C-MRP piglets had lower weight gain, growth and skeletal muscle FSR, and lower phosphorylated p70S6K1 with higher eIF4E*4E-BP1, indicative of reduced initiation of protein translation. Finally, plasma leucine concentrations were positively correlated with weight and phosphorylated p70S6K1 while negatively correlated with eIF4E*4E-BP1. In conclusion, reductions in weight gain, growth, protein turnover, skeletal muscle FSR, and initiation of protein translation with moderate macronutrient restriction in colitis are not ameliorated by probiotic supplementation. However, maintaining adequate nutrient intake during colitis preserves whole body protein metabolism, but growth remains compromised.