p-Trifluoromethyldiazirinyl-etomidate: a potent photoreactive general anesthetic derivative of etomidate that is selective for ligand-gated cationic ion channels

摘要

We synthesized the R- and S-enantiomers of ethyl 1-(1-(4-(3-((trifluoromethyl)-3H-diazirin-3-yl)phenyl)ethyl)-1H-imidazole-5-carboxylate (trifluromethyldiazirinyl-etomidate), or TFD-etomidate, a novel photoactivable derivative of the stereoselective general anesthetic etomidate (R-(2-ethyl 1-(phenylethyl)-1H-imidazole-5-carboxylate)). Anesthetic potency was similar to etomidate’s, but stereoselectivity was reversed and attenuated. Relative to etomidate, TFD-etomidate was a more potent inhibitor of the excitatory receptors, nAChR (nicotinic acetylcholine receptor) ((α1)2β1δ1γ1) and 5-HT3AR (serotonin type 3A receptor), causing significant inhibition at anesthetic concentrations. S-but not R-TFD-etomidate enhanced currents elicited from inhibitory α1β2γ2L GABAARs by low concentrations of GABA, but with a lower efficacy than R-etomidate, and site–directed mutagenesis suggests they act at different sites. [³H]TFD-etomidate photolabeled the α-subunit of the nAChR in a manner allosterically regulated by agonists and non-competitive inhibitors. TFD-etomidate’s novel pharmacology is unlike that of etomidate derivatives with photoactivable groups in the ester position, which behave like etomidate, suggesting that it will further enhance our understanding of anesthetic mechanisms.