Human natural killer cells proliferate and die in vivo more rapidly than T cells in healthy young and elderly adults

摘要

Natural killer (NK) cells are essential for health, yet little is known about human NK turnover in vivo. In both young and elderly women, all NK subsets proliferated and died more rapidly than T cells. CD56^bright NK cells proliferated rapidly, but died relatively slowly, suggesting that proliferating CD56^bright cells differentiate into CD56^dim NK cells in vivo. The relationship between CD56^dim and CD56^bright proliferating cells indicates that proliferating CD56^dim cells both self renew and are derived from proliferating CD56^bright NK cells. Our data suggests that some dying CD56^dim cells become CD16⁺CD56⁻ NK cells and that CD16⁻CD56^low NK cells respond rapidly to cellular and cytokine stimulation. We propose a model in which all NK cell subsets are in dynamic flux. About half of CD56^dim NK cells expressed CD57, which was weakly associated with low proliferation. Surprisingly, CD57 expression was associated with higher proliferation rates in both CD8⁺ and CD8⁻ T cells. Therefore, CD57 is not a reliable marker of senescent, nonproliferative T cells in vivo. NKG2A expression declined with age on both NK cells and T cells. KIR expression increased with age on T cells, but not on NK cells. Although the percentage of CD56^bright NK cells declined with age and the percentage of CD56^dim NK cells increased with age, there were no significant age-related proliferation or apoptosis differences for these two populations or for total NK cells. In vivo human NK cell turnover is rapid in both young and elderly adults.