Two Antiproliferative Triterpene Saponins from Nematostylis anthophylla from the Highlands of Central Madagascar

摘要

Investigation of the endemic Madagascan plant Nematostylis anthophylla (Rubiaceae) for antiproliferative activity against the A2780 ovarian cancer cell line led to the isolation of the known triterpene saponin randianin (>1) and the two new bioactive triterpene saponins 2″-O-acetylrandianin (>2) and 6″-O-acetylrandianin (>3). The structures of the two new compounds were elucidated based on analysis of their 1D and 2D NMR spectra, and mass spectrometric data. The three isolated triterpene saponins displayed moderate but selective antiproliferative activity, with IC50 values of 1.2, 1.7 and 2.2 μM, respectively, against the A2780 ovarian cancer, but only weak inhibition of the proliferation of A2058 melanoma and the H522 lung cancer cell lines.