Studies on Insecticidal Activities and Action Mechanism of Novel Benzoylphenylurea Candidate NK-17

摘要

Insecticidal activity of >NK-17 was evaluated both in laboratory and in field. It was found that the toxicity of >NK-17 against S. exigua was 1.93 times and 2.69 times those of hexaflumuron and chlorfluazuron respectively, and the toxicity of >NK-17 against P. xylostella was 1.36 times and 1.90 times those of hexaflumuron and chlorfluazuron respectively, and the toxicity of >NK-17 against M. separate was 18.24 times those of hexaflumuron in laboratory, and 5% >NK-17 EC at 60 g a.i ha⁻¹ can control S. exigua and P. xylostella with the best control efficiency of about 89% and over 88% respectively in Changsha and Tianjin in field. The insecticidal mechanism of >NK-17 was explored for the first time by utilizing the fluorescence polarization method. >NK-17 could bind to sulfonylurea receptor (SUR) of B. germanica with stronger affinity comparing to diflubenzuron and glibenclamide, which suggested that >NK-17 may also act on the site of SUR to inhibit the chitin synthesis in insect body and the result can well explain that >NK-17 exhibited stronger toxicity against B. germanica than diflubenzuron and glibenclamide in vivo.