The Effect of Air Exposure and Suction on Blood Cell Activation Hemolysis in an In-Vitro Cardiotomy Suction Model

摘要

Cardiopulmonary bypass (CPB) elicits a systemic inflammatory response. The cause may include surface-induced leucocyte activation and hemolysis. An in-vitro study was designed to describe the effects of both suction and an air-blood interface independently, and in combination on leucocyte and platelet activation, and hemolysis in an in-vitro model.Fresh human blood was drawn and tested under 4 different conditions including: control (A), 10 minutes of − 600 mmHg suction (B), 10 minutes of blood exposure to room air at 100 ml/min (C), and 10 min of simultaneous suction and air flow (D). Samples were analyzed by flow cytometry (platelets and leucocytes) and plasma free hemoglobin (PFHb). Leucocyte CD11b expression and platelet P-selectin (CD62P) were analyzed by flow cytometry.Compared to baseline, granulocytes were significantly activated by air (Group C, p=0.0029), and combination (Group D, p=.0123) but not by suction alone (Group B). Monocytes and platelets were not significantly activated in any group. The PFHb increased significantly in Group C (p<0.001) and Group D (p<0.001).This study suggests that the inflammatory response and associated hemolysis during CPB may be related to air exposure, which could be reduced by minimizing the air exposure of air to blood during cardiotomy suction.