首页>
美国卫生研究院文献>other
>Combining Forces - The Use of Landsat TM Satellite Imagery Soil Parameter Information and Multiplex PCR to Detect Coccidioides immitis Growth Sites in Kern County California
【2h】
Combining Forces - The Use of Landsat TM Satellite Imagery Soil Parameter Information and Multiplex PCR to Detect Coccidioides immitis Growth Sites in Kern County California
Coccidioidomycosis is a fungal disease acquired through the inhalation of spores of Coccidioides spp., which afflicts primarily humans and other mammals. It is endemic to areas in the southwestern United States, including the San Joaquin Valley portion of Kern County, California, our region of interest (ROI). Recently, incidence of coccidioidomycosis, also known as valley fever, has increased significantly, and several factors including climate change have been suggested as possible drivers for this observation. Up to date details about the ecological niche of C. immitis have escaped full characterization. In our project, we chose a three-step approach to investigate this niche: 1) We examined Landsat-5-Thematic-Mapper multispectral images of our ROI by using training pixels at a 750 m×750 m section of Sharktooth Hill, a site confirmed to be a C. immitis growth site, to implement a Maximum Likelihood Classification scheme to map out the locations that could be suitable to support the growth of the pathogen; 2) We used the websoilsurvey database of the US Department of Agriculture to obtain soil parameter data; and 3) We investigated soil samples from 23 sites around Bakersfield, California using a multiplex Polymerase Chain Reaction (PCR) based method to detect the pathogen. Our results indicated that a combination of satellite imagery, soil type information, and multiplex PCR are powerful tools to predict and identify growth sites of C. immitis. This approach can be used as a basis for systematic sampling and investigation of soils to detect Coccidioides spp.
展开▼
机译:球孢子菌病是一种通过吸入球孢子虫的孢子而获得的真菌病,主要侵害人类和其他哺乳动物。它是美国西南部地区的特有疾病,包括我们感兴趣的地区(ROI)加利福尼亚州克恩县的圣华金河谷地区。最近,球孢子菌病(也被称为谷热)的发生率显着增加,并且有人提出包括气候变化在内的多种因素可能是导致这一现象的原因。到目前为止,关于C. immitis的生态位的详细信息尚未得到完整描述。在我们的项目中,我们选择了三步方法来研究这一利基市场:1)我们通过在站点Sharktooth Hill 750 m×750 m区域使用训练像素,检查了ROI的Landsat-5-Thematic-Mapper多光谱图像确认是丙型肝炎病毒的生长地点,以实施“最大可能性分类”方案,以绘制出可能适合于支持病原体生长的位置; 2)我们使用了美国农业部的websoilsurvey数据库来获取土壤参数数据; 3)我们使用基于多重聚合酶链反应(PCR)的方法调查了加利福尼亚州贝克斯菲尔德附近23个地点的土壤样品,以检测病原体。我们的结果表明,卫星图像,土壤类型信息和多重PCR的组合是预测和识别C. Immitis生长部位的强大工具。这种方法可以用作系统采样和调查土壤以检测球孢子虫的基础。
展开▼