High-density self-contained microfluidic KOALA kits for use by everyone

摘要

Cell based assays are essential tools utilized by research labs in a wide range of fields, including cell biology, toxicology, and natural product discovery labs, however in some situations the need for cell based assays does not justify the costs of maintaining cell culture facilities and retaining skilled staff. The Kit-On-A-Lid-Assay (KOALA) technology enables accessible low-cost and pre-packageable microfluidic platforms that can be operated with minimal infrastructure or training. Here, we demonstrate and characterize high-density KOALA methods for high throughput applications, achieving an assay density comparable to that of a 384 well-plate and usability by hand with no liquid handling equipment. We show the potential for high-content screening and complex assays such as quantitative immunochemistry assays requiring multiple steps and reagents.