Colorimetric staining techniques such as immunohistochemistry (IHC), immunofluorescence (IF) and histochemistry (HC) provide useful information regarding the localization and relative amount of a molecule/substance in skin. We have developed a novel, straightforward method to assess colorimetric staining by combining features from two open-source software programs. As a proof of principle, we demonstrate the utility of this approach by analyzing changes in skin melanin deposition during the radiation-induced tanning response of Yucatan mini-pigs. This method includes a visualization step to validate the accuracy of color selection before quantitation to ensure accuracy. The data show that this method is robust and will provide a means to obtain accurate comparative analyses of staining in IHC/IF/HC samples.
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机译：比色染色技术，例如免疫组织化学（IHC），免疫荧光（IF）和组织化学（HC），提供了有关分子/物质在皮肤中的定位和相对量的有用信息。我们已经开发出了一种新颖，简单的方法，通过结合两个开源软件程序的功能来评估比色染色。作为原理的证明，我们通过分析尤卡坦小型猪在辐射诱发的晒黑反应过程中皮肤黑色素沉积的变化，证明了该方法的实用性。此方法包括可视化步骤，以在定量之前验证颜色选择的准确性以确保准确性。数据表明该方法是可靠的，并且将提供一种手段来获得IHC / IF / HC样品中染色的准确比较分析。
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