In-situ Raman spectroscopic-based microfluidic labon-a-chip platform for non-destructive and continuous characterization of Pseudomonas aeruginosa biofilms

摘要

Pseudomonas aeruginosa biofilm was cultivated and characterized in a microfluidic “lab-on-a-chip” platform coupled with confocal Raman microscopy in a nondestructive manner. Biofilm formation could be quantified by this label-free platform and correlated well to confocal laser scanning microscopy. This Raman-microfluidic platform could also discriminate biofilms at different developmental stages.