DNA Antenna Tile-Associated Deoxyribozyme Sensor with Improved Sensitivity

摘要

Some natural enzymes increase the rate of diffusion-limited reactions by facilitating substrate flow to their active sites. Inspired by this natural phenomenon, we developed a strategy for efficient substrate delivery to a deoxyribozyme (DZ) catalytic sensor. This resulted in 3- to 4-fold increase in its sensitivity and up to 9-fold improvement of the detection limit. The reported strategy can be used to enhance catalytic efficiency of diffusion-limited enzymes and to improve sensitivity of enzyme-based biosensors.