The anaphase promoting complex/cyclosome (APC/C) is a large multi-subunit ubiquitin ligase that triggers the metaphase-to-anaphase transition in the cell cycle by targeting the substrates Cyclin B and securin for destruction. APC/C activity towards these two key substrates requires the co-activator Cdc20. To ensure that cells enter mitosis and partition their duplicated genome with high accuracy, APC/CCdc20 activity must be tightly controlled. Here, we discuss the mechanisms that regulate APC/CCdc20 activity both prior to and during mitosis. We focus our discussion primarily on the chromosomal pathways that both accelerate and delay APC/C activation by targeting Cdc20 to opposing fates. The findings discussed provide an overview of how cells control the activation of this major cell cycle regulator to ensure both accurate and timely cell division.
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机译:后期促进复合物/环体(APC / C)是一个大型的多亚基泛素连接酶,通过靶向底物Cyclin B和securin进行破坏,从而触发了细胞周期的中期到后期过渡。针对这两个关键底物的APC / C活性需要辅助活化剂Cdc20。为了确保细胞进入有丝分裂并高精度地复制其复制的基因组,必须严格控制APC / C Cdc20 sup>活性。在这里,我们讨论有丝分裂之前和期间调节APC / C Cdc20 sup>活性的机制。我们的讨论主要集中在通过将Cdc20靶向相反的命运而加速和延迟APC / C活化的染色体途径上。讨论的发现概述了细胞如何控制该主要细胞周期调节剂的活化,以确保准确而及时的细胞分裂。
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