Information-Theoretic Approach and Fundamental Limits of Resolving Two Closely-Timed Neuronal Spikes in Mouse Brain Calcium Imaging

摘要

Objective:Although optical imaging of neurons using fluorescent genetically encoded calcium sensors has enabled large-scale in vivo experiments, the sensors’ slow dynamics often blur closely-timed action potentials into indistinguishable transients. While several previous approaches have been proposed to estimate the timing of individual spikes, they have overlooked the important and practical problem of estimating inter-spike-interval (ISI) for overlapping transients.