Single-cell Time-lapse Reactive Oxygen Species Detection in E. coli

摘要

We have developed single-cell, time-lapse assays to detect reactive oxygen species (ROS) in live E. coli. The assay utilizes flow systems on a fluorescence microscopy to correlate symptoms aroused from biological or chemical perturbations with the in situ detection of ROS. ROS is detected by fluorogenic dyes that accumulate inside the cell, allowing detection of ROS in single cells in both homogeneous and heterogeneous samples using CellROX Green and Amplex Red/APEX2.