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The physicochemical and biomechanical profile of forsterite and its osteogenic potential of mesenchymal stromal cells

机译:镁橄榄石的理化和生物力学特征及其间充质基质细胞的成骨潜能

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摘要

It has been demonstrated that nanocrystalline forsterite powder synthesised using urea as a fuel in sol-gel combustion method had produced a pure forsterite (FU) and possessed superior bioactive characteristics such as bone apatite formation and antibacterial properties. In the present study, 3D-scaffold was fabricated using nanocrystalline forsterite powder in polymer sponge method. The FU scaffold was used in investigating the physicochemical, biomechanics, cell attachment, in vitro biocompatibility and osteogenic differentiation properties. For physicochemical characterisation, Fourier-transform infrared spectroscopy (FTIR), Energy dispersive X-ray (EDX), X-ray diffraction (XRD), Raman spectroscopy, X-ray photoemission spectrometer (XPS) and Brunauer-Emmett-Teller (BET) were used. FTIR, EDX, XRD peaks and Raman spectroscopy demonstrated correlating to FU. The XPS confirmed the surface chemistry associating to FU. The BET revealed FU scaffold surface area of 12.67 m2/g and total pore size of 0.03 cm3/g. Compressive strength of the FU scaffold was found to be 27.18 ± 13.4 MPa. The human bone marrow derived mesenchymal stromal cells (hBMSCs) characterisation prior to perform seeding on FU scaffold verified the stromal cell phenotypic and lineage commitments. SEM, confocal images and presto blue viability assay suggested good cell attachment and proliferation of hBMSCs on FU scaffold and comparable to a commercial bone substitutes (cBS). Osteogenic proteins and gene expression from day 7 onward indicated FU scaffold had a significant osteogenic potential (p<0.05), when compared with day 1 as well as between FU and cBS. These findings suggest that FU scaffold has a greater potential for use in orthopaedic and/or orthodontic applications.
机译:已经证明,使用尿素作为燃料在溶胶-凝胶燃烧法中合成的纳米晶镁橄榄石粉末已经产生了纯镁橄榄石(FU),并且具有优异的生物活性特征,例如骨磷灰石形成和抗菌性能。在本研究中,使用纳米晶镁橄榄石粉末在聚合物海绵法中制备了3D支架。 FU支架用于研究理化,生物力学,细胞附着,体外生物相容性和成骨分化特性。对于物理化学表征,傅里叶变换红外光谱(FTIR),能量色散X射线(EDX),X射线衍射(XRD),拉曼光谱,X射线光发射光谱仪(XPS)和Brunauer-Emmett-Teller(BET)被使用。 FTIR,EDX,XRD峰和拉曼光谱证明与FU有关。 XPS证实了表面化学与FU有关。 BET显示FU支架表面积为12.67 m 2 / g,总孔径为0.03 cm 3 / g。发现FU支架的抗压强度为27.18±13.4MPa。在FU支架上播种之前,人骨髓来源的间充质基质细胞(hBMSCs)的特征验证了基质细胞的表型和谱系承诺。 SEM,共聚焦图像和蓝色活度测定表明,hBMSC在FU支架上具有良好的细胞附着和增殖能力,可与商业骨替代品(cBS)媲美。从第7天开始,成骨蛋白和基因表达表明,与第1天以及在FU和cBS之间相比,FU支架具有显着的成骨潜能(p <0.05)。这些发现表明,FU支架在骨科和/或正畸应用中具有更大的潜力。

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