Inhibition of B6RV2 Leukemia Growth by Immunization with Purified Unique Antigen

摘要

Monoclonal antibody (mAb) NU7‐99 reacted with only B6RV2 cells, not with 28 other leukemia cell lines, fibroblasts or normal tissues. Biochemical analyses of the unique antigen on B6RV2 cells that reacted with NU7–99 mAb indicated its relationship to xenotropic murine leukemia virus gp70. The antigen that reacted with NU7‐99 mAb was extracted from the surface of B6RV2 cells with n‐butanol and purified by ion‐exchange chromatography and affinity chromatography. Growth of B6RV2 tumors in semi‐syngeneic mice was inhibited by immunization of the mice with a purified preparation of this unique antigen.