We demonstrate the use of a compressive sampling algorithm for on-chip fluorescent imaging of sparse objects over an ultra-large field-of-view (>8 cm2) without the need for any lenses or mechanical scanning. In this lensfree imaging technique, fluorescent samples placed on a chip are excited through a prism interface, where the pump light is filtered out by total internal reflection after exciting the entire sample volume. The emitted fluorescent light from the specimen is collected through an on-chip fiber-optic faceplate and is delivered to a wide field-of-view opto-electronic sensor array for lensless recording of fluorescent spots corresponding to the samples. A compressive sampling based optimization algorithm is then used to rapidly reconstruct the sparse distribution of fluorescent sources to achieve ~10 µm spatial resolution over the entire active region of the sensor-array, i.e., over an imaging field-of-view of >8 cm2. Such a wide-field lensless fluorescent imaging platform could especially be significant for high-throughput imaging cytometry, rare cell analysis, as well as for micro-array research.
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机译:我们演示了使用压缩采样算法对超大视野(> 8 cm 2 sup>)上的稀疏对象进行片上荧光成像的过程,而无需任何透镜或机械扫描。在这种无透镜成像技术中,放置在芯片上的荧光样品通过棱镜界面被激发,在激发整个样品体积之后,泵浦光被全内反射滤除。样品发出的荧光通过芯片上的光纤面板收集,然后传送到宽视野的光电传感器阵列,以无透镜方式记录与样品对应的荧光点。然后使用基于压缩采样的优化算法快速重建荧光源的稀疏分布,以在传感器阵列的整个有效区域上(即在大于8 cm的成像视场上)实现约10 µm的空间分辨率 2 sup>。这样的广角无透镜荧光成像平台对于高通量成像细胞计数,稀有细胞分析以及微阵列研究尤其重要。
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