We present a two-photon microscope that images the full extent of murine cortex with an objective-limited spatial resolution across an 8 mm by 10 mm field. The lateral resolution is approximately 1 µm and the maximum scan speed is 5 mm/ms. The scan pathway employs large diameter compound lenses to minimize aberrations and performs near theoretical limits. We demonstrate the special utility of the microscope by recording resting-state vasomotion across both hemispheres of the murine brain through a transcranial window and by imaging histological sections without the need to stitch.
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机译：我们提出了一个两光子显微镜，可以在8mm x 10mm的视野中以目标有限的空间分辨率成像鼠皮质的整个范围。横向分辨率约为1 µm，最大扫描速度为5 mm / ms。扫描路径采用大直径复合透镜以最大程度地减少像差，并接近理论极限。我们通过横穿颅窗记录鼠脑两个半球的静止状态血管运动，以及无需缝合就可以对组织切片进行成像，从而证明了显微镜的特殊用途。
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