Hydrophobic protein from soybean (HPS) is present in soybean dust and is an allergen (Gly m 1) that causes asthma in allergic individuals. Past studies have shown that HPS occurs on the seed surface. To determine the microscopic localization of HPS during seed development, monoclonal antibodies to HPS were used to visualize the protein by fluorescence and transmission electron microscopy. Seed coat and endocarp sections were also examined for pectin, cellulose, callose, starch, and protein by histochemical staining. HPS is present in the endocarp epidermal cells at 18 to 28 days post anthesis. At later stages of seed development, HPS occurs in extracellular secretions that accumulate unevenly on the endocarp epidermis and seed surface. HPS is synthesized by the endocarp epidermis and deposited on the seed surface as part of a heterogeneous matrix.
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机译：来自大豆的疏水蛋白（HPS）存在于大豆粉尘中，是一种过敏原（Gly m 1），可引起过敏性个体哮喘。过去的研究表明，HPS发生在种子表面。为了确定种子发育过程中HPS的微观定位，使用了针对HPS的单克隆抗体通过荧光和透射电子显微镜观察蛋白质。还通过组织化学染色检查了种皮和果皮切片的果胶，纤维素，call质，淀粉和蛋白质。花后18到28天，HPS存在于果皮表皮细胞中。在种子发育的后期，HPS发生在胞外分泌物中，这些分泌物不均匀地积累在内果皮表皮和种子表面上。 HPS由果皮表皮合成，并作为异质基质的一部分沉积在种子表面。
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