Ozone (O3) has attracted much attention because of its key role in human health and disease, and its detection is of great importance for various biochemical studies as well as environmental evaluation. Here we develop a simple and practical spectroscopic off-on O3 probe based on resorufin and the specific reaction of but-3-enyl with O3. The probe shows an extremely low background spectroscopic signal, but reacts with O3 producing a distinct color and fluorescence change. The detection limit of the probe for O3 is 5.9 nM, which corresponds to an ozone concentration of 0.056 mg m−3 in air in this study and is lower than the international ambient air quality standard of 0.1 mg m−3. More importantly, the proposed probe is worth popularizing, and its applicability has been successfully demonstrated on both the determination of O3 in real ambient air samples and the imaging of O3 in biological cells.
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机译：臭氧（O3）在人类健康和疾病中起着关键作用，因此受到了广泛的关注，其检测对于各种生化研究和环境评估都具有重要意义。在这里，我们基于试卤灵和丁-3-烯基与O3的特异性反应，开发了一种简单实用的光谱关闭O3探针。探针显示出极低的背景光谱信号，但与O3反应产生明显的颜色和荧光变化。探针中O3的检出限为5.9 nM，相当于本研究中空气中的臭氧浓度为0.056 mg m −3 ，低于国际环境空气质量标准0.1 mg m -3 。更重要的是，该探针值得推广，在实际环境空气样品中O3的测定和生物细胞中O3的成像方面都已成功证明了其适用性。
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