Structural basis for ion selectivity revealed by high-resolution crystal structure of Mg2+ channel MgtE

摘要

Magnesium is the most abundant divalent cation in living cells and is crucial to several biological processes. MgtE is a Mg²⁺ channel distributed in all domains of life that contributes to the maintenance of cellular Mg²⁺ homeostasis. Here we report the high-resolution crystal structures of the transmembrane domain of MgtE, bound to Mg²⁺, Mn²⁺ and Ca²⁺. The high-resolution Mg²⁺-bound crystal structure clearly visualized the hydrated Mg²⁺ ion within its selectivity filter. Based on those structures and biochemical analyses, we propose a cation selectivity mechanism for MgtE in which the geometry of the hydration shell of the fully hydrated Mg²⁺ ion is recognized by the side-chain carboxylate groups in the selectivity filter. This is in contrast to the K⁺-selective filter of KcsA, which recognizes a dehydrated K⁺ ion. Our results further revealed a cation-binding site on the periplasmic side, which regulate channel opening and prevents conduction of near-cognate cations.