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Rotating magnetic field as tool for enhancing enzymes properties - laccase case study

机译:旋转磁场作为增强酶性能的工具-漆酶案例研究

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摘要

The aim of this study was to analyse the effect of rotating magnetic field (RMF) exposition on the fungal laccase catalytic properties. The results obtained in the study revealed that RMF may positively alter the laccase activity. A significant increase in activities of 11%, 11%, and 9% were observed at 10 Hz, 40 Hz and 50 Hz, respectively. Exposure of laccase to the rotating magnetic field resulted in its increased activity at broader pH range and a slight shift in optimum pH from 4.0 to 4.5 at RMF with frequency 20 Hz. The results show that the enzyme activity, stability, and optimum pH can be significantly altered depending on the characteristic of the applied RMF. Application of rotating magnetic field opens a new way for controlling and directions of enzyme-based bioprocessing.
机译:这项研究的目的是分析旋转磁场(RMF)暴露对真菌漆酶催化性能的影响。研究获得的结果表明,RMF可能会积极改变漆酶的活性。在10 Hz,40 Hz和50 Hz处分别观察到11%,11%和9%的活性显着增加。将漆酶暴露在旋转磁场中会导致其在更宽的pH范围内增加活性,并且在20 MHz的RMF条件下,最佳pH从4.0到4.5略有变化。结果表明,取决于所施加的RMF的特性,酶活性,稳定性和最适pH可以显着改变。旋转磁场的应用为基于酶的生物加工的控制和方向开辟了一条新途径。

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