The ability of Anopheles gambiae mosquitoes to bite through a permethrin-treated net and the consequences for their fitness

摘要

Insecticide-treated bed-nets (ITNs) control malaria by keeping mosquitoes from reaching people sleeping under a net and by killing mosquitoes. Most tests of ITNs consider their overall epidemiological outcome without considering the different behaviors underlying their effects. Here we consider one of these behaviors: that mosquitoes can bite through the net if its user is touching it. We assayed the ability of an insecticide-sensitive strain of the mosquito Anopheles gambiae to bite through a permethrin-treated or an untreated net, and their subsequent survival and fecundity. Despite the irritancy of permethrin, 71% of the mosquitoes took blood through the ITN (vs. 99% through the untreated net). The ITN reduced the time spent biting, the blood-meal size and the fecundity, and it killed about 15% of the mosquitoes within 24 hours of feeding (vs. 5% on the untreated net). However, the mosquito’s survival was much higher than what we found in WHO cone assays, suggesting that the bloodmeal increased the mosquito’s resistance to the insecticide. Thus, our results suggest that the irritancy and the toxicity of ITNs are reduced when mosquitoes contact and feed on their host, which will affect our understanding of the personal and community protection offered by the ITNs.