Different Innate Signatures Induced in Human Monocyte-derived Dendritic Cells by Wild-Type Dengue 3 Virus Attenuated but Reactogenic Dengue 3 Vaccine Virus or Attenuated Nonreactogenic Dengue 1–4 Vaccine Virus Strains

摘要

DNA microarrays were used to assess the innate gene signature in human myeloid dendritic cells infected with chimeric dengue 1–4 vaccines, a wild-type dengue 3 virus, or a classically attenuated serotype 3 vaccine shown to be reactogenic in humans. We observed a very reproducible signature for each of the 4 chimeric dengue vaccines, involving stimulation of type I interferon and associated genes, together with genes encoding chemokines and other mediators involved in the initiation of adaptive responses. In contrast, wild-typeDEN3 virus induced a predominantly inflammatory profile, while the reactogenic attenuated serotype 3 vaccine appeared to induce a blunted response.