Antimicrobial Blue Light Inactivation of Gram-Negative Pathogens in Biofilms: In Vitro and In Vivo Studies

摘要

>Background. Biofilms affect >80% bacterial infections in human and are usually difficult to eradicate because of their inherent drug resistance.>Methods. We investigated the effectiveness of antimicrobial blue light (aBL) (wavelength, 415 nm) for inactivating Acinetobacter baumannii or Pseudomonas aeruginosa biofilms in 96-well microplates or infected mouse burn wounds.>Results. In vitro, in 96-well microplates, exposure of 24-hour-old and 72-hour-old A. baumannii biofilms to 432 J/cm² aBL resulted in inactivation of 3.59 log10 and 3.18 log10 colony-forming units (CFU), respectively. For P. aeruginosa biofilms, similar levels of inactivation—3.02 log10 and 3.12 log10 CFU, respectively—were achieved. In mouse burn wounds infected with 5 × 10⁶ CFU of A. baumannii, approximately 360 J/cm² and 540 J/cm² aBL was required to inactivate 3 log10 CFU in biofilms when delivered 24 and 48 hours, respectively, after bacterial inoculation. High-performance liquid chromatography analysis revealed the presence of endogenous porphyrins in both A. baumannii and P. aeruginosa. TUNEL assay detected no apoptotic cells in aBL-irradiated mouse skin at up to 24 hours after aBL exposure (540 J/cm²).>Conclusions. aBL has antimicrobial activity in biofilms of A. baumannii and P. aeruginosa and is a potential therapeutic approach for biofilm-related infections.