A kinetic analysis of the inhibition of FOX-4 β-lactamase a plasmid-mediated AmpC cephalosporinase by monocyclic β-lactams and carbapenems

摘要

ObjectivesClass C β-lactamases are prevalent among Enterobacteriaceae; however, these enzymes are resistant to inactivation by commercially available β-lactamase inhibitors. In order to find novel scaffolds to inhibit class C β-lactamases, the comparative efficacy of monocyclic β-lactam antibiotics (aztreonam and the siderophore monosulfactam ), the bridged monobactam β-lactamase inhibitor , and carbapenems (imipenem, meropenem, doripenem and ertapenem) were tested in kinetic assays against FOX-4, a plasmid-mediated class C β-lactamase (pmAmpC).