Helioscopianoids A–Q bioactive jatrophane diterpenoid esters from Euphorbia helioscopia

摘要

The EtOH extracts of the whole plants of Euphorbia helioscopia afforded 17 new jatrophane diterpenoid esters, helioscopianoids A–Q (>1—>17), along with eight known compounds (>18–>25). Their structures were elucidated by extensive spectroscopic methods and Mo2(OAc)4-induced ECD analysis, and the structures of compounds >1, >2, and >7 were confirmed by X-ray crystallography. Compounds >1–>17 were evaluated for inhibitory effects on P-glycoprotein (P-gp) in an adriamycin (ADM)-resistant human breast adenocarcinoma cell line (MCF-7/ADR) and neuroprotective effects against serum deprivation-induced and rotenone-induced PC12 cell damage. Compounds >8 and >16 increased the accumulation of ADM in MCF-7/ADR cells by approximately 3-fold at a concentration of 20 μmol/L. Compound >8 could attenuate rotenone-induced PC12 cell damage, and compounds >2, >8, and >12 showed neuroprotective activities against serum deprivation-induced PC12 cell damage.