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Evaluation of Human Enteric Viruses in Surface Water and Drinking Water Resources in Southern Ghana

机译:加纳南部地表水和饮用水资源中人类肠道病毒的评估

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摘要

An estimated 884 million people worldwide do not have access to an improved drinking water source, and the microbial quality of these sources is often unknown. In this study, a combined tangential flow, hollow fiber ultrafiltration (UF), and real-time PCR method was applied to large volume (100 L) groundwater (N = 4), surface water (N = 9), and finished (i.e., receiving treatment) drinking water (N = 6) samples for the evaluation of human enteric viruses and bacterial indicators. Human enteric viruses including norovirus GI and GII, adenovirus, and polyomavirus were detected in five different samples including one groundwater, three surface water, and one drinking water sample. Total coliforms and Escherichia coli assessed for each sample before and after UF revealed a lack of correlation between bacterial indicators and the presence of human enteric viruses.
机译:全世界估计有8.84亿人无法获得改善的饮用水源,而这些水源的微生物质量通常是未知的。在这项研究中,将切向流,中空纤维超滤(UF)和实时PCR的组合方法应用于大体积(100 L)地下水(N = 4),地表水(N = 9),并最终处理(即(接受治疗)饮用水(N = 6)样本,用于评估人肠病毒和细菌指标。在五个不同的样本中检测到了人类肠道病毒,包括诺如病毒GI和GII,腺病毒和多瘤病毒,其中包括一个地下水,三个地表水和一个饮用水样本。超滤前后每个样品的总大肠菌群和大肠埃希菌评估表明细菌指标与人类肠道病毒的存在之间缺乏相关性。

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