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Multilocus Sequence Typing Pulsed-Field Gel Electrophoresis and fla Short Variable Region Typing of Clonal Complexes of Campylobacter jejuni Strains of Human Bovine and Poultry Origins in Luxembourg

机译:卢森堡人牛和家禽空肠弯曲杆菌菌株的克隆复合体的多基因座序列分型脉冲场凝胶电泳和fla短可变区分型

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摘要

Campylobacter jejuni is the most common cause of bacterial gastroenteritis in Luxembourg, with a marked seasonal peak during summer. The majority of these infections are thought to be sporadic, and the relative contribution of potential sources and reservoirs is still poorly understood. We monitored human cases from June to September 2006 (n = 124) by molecular characterization of isolates with the aim of rapidly detecting temporally related cases. In addition, isolates from poultry meat (n = 36) and cattle cecal contents (n = 48) were genotyped for comparison and identification of common clusters between veterinary and human C. jejuni populations. A total of 208 isolates were typed by sequencing the fla short variable region, macrorestriction analysis resolved by pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). We observed a high diversity of human strains during a given summer season. Poultry and human isolates had a higher diversity of sequence types than isolates of bovine origin, for which clonal complexes CC21 (41.6%) and CC61 (18.7%) were predominant. CC21 was also the most common complex found among human isolates (21.8%). The substantial concordance between PFGE and MLST results for this last group of strains suggests that they are clonally related. Our study indicates that while poultry remains an important source, cattle could be an underestimated reservoir of human C. jejuni cases. Transmission mechanisms of cattle-specific strains warrant further investigation.
机译:空肠弯曲菌是卢森堡细菌性肠胃炎的最常见原因,夏季有明显的季节性高峰。这些感染中的大多数被认为是零星的,对潜在来源和储库的相对贡献仍然知之甚少。我们通过分离株的分子表征监测了2006年6月至2006年9月的人类病例(n = 124),目的是迅速发现与时间相关的病例。此外,对禽肉(n = 36)和牛盲肠内容物(n = 48)的分离株进行了基因分型,以比较和鉴定兽医和空肠弯曲杆菌种群之间的常见簇。通过对fla短可变区进行测序,通过脉冲场凝胶电泳(PFGE)解决的宏观限制性分析和多基因座序列分型(MLST),对总共208个分离株进行分型。在给定的夏季,我们观察到了人类菌株的高度多样性。家禽和人类分离株的序列类型多样性比牛来源分离株的序列类型更高,其中以克隆复合物CC21(41.6%)和CC61(18.7%)为主导。 CC21也是人类分离株中最常见的复合物(21.8%)。对于最后一组菌株,PFGE和MLST结果之间的基本一致性表明它们是克隆相关的。我们的研究表明,尽管家禽仍然是重要来源,但牛可能是人类空肠弯曲杆菌病例的一个被低估的库。牛特异性菌株的传播机制值得进一步研究。

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