首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Molecular Analysis of a Saccharomyces cerevisiae Mutant with Improved Ability To Utilize Xylose Shows Enhanced Expression of Proteins Involved in Transport Initial Xylose Metabolism and the Pentose Phosphate Pathway
【2h】

Molecular Analysis of a Saccharomyces cerevisiae Mutant with Improved Ability To Utilize Xylose Shows Enhanced Expression of Proteins Involved in Transport Initial Xylose Metabolism and the Pentose Phosphate Pathway

机译:具有改进的利用木糖能力的啤酒酵母突变体的分子分析显示参与运输初始木糖代谢和磷酸戊糖途径的蛋白质表达增强。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。
获取外文期刊封面目录资料

摘要

Differences between the recombinant xylose-utilizing Saccharomyces cerevisiae strain TMB 3399 and the mutant strain TMB 3400, derived from TMB 3399 and displaying improved ability to utilize xylose, were investigated by using genome-wide expression analysis, physiological characterization, and biochemical assays. Samples for analysis were withdrawn from chemostat cultures. The characteristics of S. cerevisiae TMB 3399 and TMB 3400 grown on glucose and on a mixture of glucose and xylose, as well as of S. cerevisiae TMB 3400 grown on only xylose, were investigated. The strains were cultivated under chemostat conditions at a dilution rate of 0.1 h−1, with feeds consisting of a defined mineral medium supplemented with 10 g of glucose liter−1, 10 g of glucose plus 10 g of xylose liter−1 or, for S. cerevisiae TMB 3400, 20 g of xylose liter−1. S. cerevisiae TMB 3400 consumed 31% more xylose of a feed containing both glucose and xylose than S. cerevisiae TMB 3399. The biomass yields for S. cerevisiae TMB 3400 were 0.46 g of biomass g of consumed carbohydrate−1 on glucose and 0.43 g of biomass g of consumed carbohydrate−1 on xylose. A Ks value of 33 mM for xylose was obtained for S. cerevisiae TMB 3400. In general, the percentage error was <20% between duplicate microarray experiments originating from independent fermentation experiments. Microarray analysis showed higher expression in S. cerevisiae TMB 3400 than in S. cerevisiae TMB 3399 for (i) HXT5, encoding a hexose transporter; (ii) XKS1, encoding xylulokinase, an enzyme involved in one of the initial steps of xylose utilization; and (iii) SOL3, GND1, TAL1, and TKL1, encoding enzymes in the pentose phosphate pathway. In addition, the transcriptional regulators encoded by YCR020C, YBR083W, and YPR199C were expressed differently in the two strains. Xylose utilization was, however, not affected in strains in which YCR020C was overexpressed or deleted. The higher expression of XKS1 in S. cerevisiae TMB 3400 than in TMB 3399 correlated with higher specific xylulokinase activity in the cell extracts. The specific activity of xylose reductase and xylitol dehydrogenase was also higher for S. cerevisiae TMB 3400 than for TMB 3399, both on glucose and on the mixture of glucose and xylose.
机译:通过使用全基因组表达分析,生理学表征和生化分析,研究了利用木糖的酿酒酵母菌株TMB 3399与衍生自TMB 3399的突变菌株TMB 3400之间的差异,这些菌株显示出提高的木糖利用能力。从化学恒温培养物中取出用于分析的样品。研究了在葡萄糖以及在葡萄糖和木糖的混合物上生长的啤酒酵母TMB 3399和TMB 3400,以及仅在木糖上生长的啤酒酵母TMB 3400的特性。菌株在恒化条件下以0.1 h -1 的稀释率进行培养,饲料中加入限定的矿质培养基,并添加10 g葡萄糖升 -1 ,10克葡萄糖加10克木糖升 -1 ,或者对于酿酒酵母TMB 3400,则取20克木糖升 -1 。啤酒酵母TMB 3400消耗的葡萄糖和木糖含量均比啤酒酵母TMB 3399高31%。啤酒酵母TMB 3400的生物质产量为0.46 g生物质g消耗的碳水化合物 -1 和木糖上0.43 g的生物质g消耗的碳水化合物 -1 。对于酿酒酵母TMB 3400,木糖的Ks值为33 mM。通常,源自独立发酵实验的重复微阵列实验之间的百分比误差<20%。微阵列分析显示:(i)编码己糖转运蛋白的HXT5在酿酒酵母TMB 3400中的表达高于在酿酒酵母TMB 3399中的表达。 (ii)XKS1,编码木酮糖激酶,一种参与木糖利用的初始步骤之一的酶; (iii)SOL3,GND1,TAL1和 TKL1 ,它们编码磷酸戊糖途径中的酶。另外,由YCR020C,YBR083W和YPR199C编码的转录调节子在两种菌株中表达不同。但是,在YCR020C过表达或缺失的菌株中,木糖的利用不受影响。 XKS1 S中的较高表达。啤酒酵母TMB 3400比TMB 3399具有更高的细胞抽提物中木糖激酶活性。木糖还原酶和木糖醇脱氢酶的比活性对于 S也较高。在葡萄糖以及葡萄糖和木糖的混合物上,啤酒酵母TMB 3400比TMB 3399更好。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号