Dehydrodivanillin (DDV; 0.15 g/liter) was biodegradable at 37°C under strictly anaerobic conditions by microflora from cow rumen fluid to the extent of 25% within 2 days in a yeast extract medium. The anaerobes were acclimated on DDV for 2 weeks, leading to DDV-degrading microflora with rates of degradation eight times higher than those initially. Dehydrodivanillic acid and vanillic acid were detected in an ethylacetate extract of a DDV-enriched culture broth by thin-layer, gas, and high-performance liquid chromatographies and by mass spectrometry.
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机译:脱氢香兰素(DDV; 0.15 g /升)在严格厌氧条件下,可在37℃下通过酵母菌培养基中的微生物从牛瘤胃液中以25%的程度被微生物降解。厌氧菌在DDV上适应2周,导致DDV降解的菌群,降解速率比最初的高八倍。通过薄层,气相和高效液相色谱法以及质谱法,在富含DDV的培养液的乙酸乙酯提取物中检测到了脱氢二香草酸和香草酸。
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