A method has been developed for measurement of denitrification activity in sediments by application of the acetylene inhibition technique. Acetylene-saturated water was injected, at close intervals, into sediment cores which were then incubated for a few hours at the in situ temperature. Frozen segments of the cores were assayed for accumulation of N2O by a combined gas extraction and detection system. The segments were thawed under a stream of helium from which N2O (and other gases) was collected in a liquid N2 trap, and the quantity of N2O was measured by gas chromatography. The maximum rate of denitrification in a coastal marine sediment was 35 nmol of N per cm3 of sediment per day at 2.5°C, and the rate of denitrification for the total sediment was 0.99 nmol of N per m2 per day.
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机译：已经开发出一种通过应用乙炔抑制技术来测量沉积物中反硝化活性的方法。将乙炔饱和水以近乎固定的间隔注入沉积物岩心中，然后在原位温度下孵育几个小时。通过组合的气体提取和检测系统，对岩心的冰冻段进行了N2O累积测定。在氦流下将这些段解冻，然后在液态氮气收集器中收集N2O（和其他气体），并通过气相色谱法测量N2O的量。在2.5°C下，沿海海洋沉积物的最大反硝化速率为每天35 nmol / cm 3 沉积物，总沉积物的反硝化速率为0.99 nmol N / m每天 2 。
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