A biological safety cabinet was tested to determine the effect of crossdrafts (such as those created by normal laboratory activity or ventilation) upon the ability of the cabinet to protect both experiments and investigators. A simple crossdraft, controllable from 50 to 200 feet per min (fpm; 15.24 to 60.96 m/min), was created across the face of the unit. Modifications of standardized procedures involving controlled bacterial aerosol challenges provided stringent test conditions. Results indicated that, as the crossflow velocities exceeded 100 fpm, the ability of the cabinet to protect either experiments or investigators decreased logarithmically with increasing crossdraft speed. Because 100 fpm is an airspeed easily achieved by some air conditioning and heating vents (open windows and doorways may create velocities far in excess of 200 fpm), the proper placement of a biological safety cabinet within the laboratory--away from such disruptive air currents--is essential to satisfactory cabinet performance.
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机译:对生物安全柜进行了测试,以确定交叉通风(例如由正常实验室活动或通风产生的交叉通风)对安全柜保护实验和研究人员的能力的影响。在设备的整个表面上创建了一个简单的横风,可控制在每分钟50到200英尺(fpm; 15.24到60.96 m / min)之间。涉及控制细菌气溶胶挑战的标准化程序的修改提供了严格的测试条件。结果表明,当错流速度超过100 fpm时,箱体保护实验或研究人员的能力随错流速度的增加而对数减小。由于某些空调和通风孔很容易达到100 fpm的空速(打开的窗户和门口可能会产生超过200 fpm的速度),因此在实验室内正确放置生物安全柜-远离这种破坏性气流-对于获得令人满意的机柜性能至关重要。
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