Aliphatic Hydrocarbons of Cladosporium resinae Cultured on Glucose Glutamic Acid and Hydrocarbons

摘要

The carbon source markedly influenced the qualitative and quantitative composition of cellular hydrocarbons in Cladosporium resinae. Total lipid and hydrocarbon content was greater in cells grown on n-alkanes than in cells grown on glucose or glutamic acid. Glucose-grown cells contained a spectrum of aliphatic hydrocarbons from C7 to C36; pristane and n-hexadecane comprised 98% of the total. Cells grown on glutamic acid contained C7 to C23 hydrocarbons; n-tridecane, n-tetradecane, n-hexadecane, and pristane made up 74% of the total. n-Decane-grown cells yielded C8 to C32 compounds, and n-hexadecane (96%) was the major hydrocarbon. Cells grown on individual n-alkanes from C11 to C15 all contained C11 to C28 hydrocarbons, and cells grown on n-hexadecane contained C11 to C32 hydrocarbons. In n-undecane-grown cells, n-hexadecane and pristane made up 92% of the total, but in cells grown on C12 to C16 n-alkanes the major cellular hydrocarbon was the one on which the cells were grown. This suggests that cells cultured on n-alkanes of C12 or longer accumulate n-alkanes prior to oxidizing them.