A rapid sensitive method for the detection of viable bacterial cells is described in which P32 as inorganic orthophosphate is used to label the cells. Factors affecting the uptake of P32 by cells as well as the sensitivity of the method have been explored with suspensions of Aerobacter aerogenes. The uptake of P32O4 is dependent on several factors. Of various incubation media tested, one composed of 0.005 m KCl, 0.002 m MgSO4 and 10 mg/ml of glucose was found to best stimulate the uptake of the tracer. Incubation time and temperature and level of isotope and of unlabeled P also affected uptake. Labeled cells were collected on a membrane filter for measurement of radioactivity. Under optimal conditions, as few as 23 viable cells per milliliter were detected in 1 hr with 95% confidence.
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机译：描述了一种检测活细菌细胞的快速灵敏方法，其中使用P 32 作为无机正磷酸盐来标记细胞。研究了产气杆菌悬浮液对细胞摄取P 32 的影响因素以及该方法的敏感性。 P 32 O4的吸收取决于几个因素。在测试的各种温育培养基中，发现一种由0.005 m KCl，0.002 m MgSO4和10 mg / ml葡萄糖组成的培养基可以最好地刺激示踪剂的吸收。孵育时间，温度以及同位素和未标记P的水平也会影响摄取。将标记的细胞收集在膜滤器上以测量放射性。在最佳条件下，在1小时内以95％的置信度检测到每毫升只有23个活细胞。
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