首页> 美国卫生研究院文献>Biochemical Journal >Species differences in the occurrence of copper-metallothionein in the particulate fractions of the liver of copper-loaded animals.
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Species differences in the occurrence of copper-metallothionein in the particulate fractions of the liver of copper-loaded animals.

机译:负载铜的动物肝脏的微粒部分中铜金属硫蛋白的发生存在物种差异。

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摘要

Large amounts of Cu-metallothionein were obtained by 2-mercaptoethanol and sodium dodecyl sulphate extractions of the particulate fractions of the liver of pigs given high-Cu2+ diets or rats injected with Cu2+. Three isoproteins were purified from pig liver and characterized on the basis of their physicochemical properties, metal content and amino acid composition. No such pool of Cu-metallothionein was present in the liver of Cu2+-loaded sheep or of rats given Cu2+-supplemented diets.
机译:通过2-巯基乙醇和十二烷基硫酸钠对高Cu2 +日粮或注射Cu2 +的猪的肝脏的微粒部分进行萃取,可以获得大量的Cu-金属硫蛋白。从猪肝中纯化出三种同蛋白,并根据其理化性质,金属含量和氨基酸组成对其进行表征。负载铜2+的绵羊或补充铜2+饮食的大鼠的肝脏中不存在这样的铜金属硫蛋白库。

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