Creation and validation of a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells

摘要

BackgroundCloning vectors capable of retroviral transduction have enabled stable gene overexpression in numerous mitotic cell lines. However, the relatively small number of feasible restriction enzyme sequences in their cloning sites can hinder successful generation of overexpression constructs if these sequences are also present in the target cDNA insert.