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The transcriptome of the novel dinoflagellate Oxyrrhis marina (Alveolata: Dinophyceae): response to salinity examined by 454 sequencing

机译:新颖的鞭毛藻(Oxyrrhis marinaAlveolata:Dinophyceae)的转录组:通过454测序检测对盐度的响应

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摘要

BackgroundThe heterotrophic dinoflagellate Oxyrrhis marina is increasingly studied in experimental, ecological and evolutionary contexts. Its basal phylogenetic position within the dinoflagellates make O. marina useful for understanding the origin of numerous unusual features of the dinoflagellate lineage; its broad distribution has lent O. marina to the study of protist biogeography; and nutritive flexibility and eurytopy have made it a common lab rat for the investigation of physiological responses of marine heterotrophic flagellates. Nevertheless, genome-scale resources for O. marina are scarce. Here we present a 454-based transcriptome survey for this organism. In addition, we assess sequence read abundance, as a proxy for gene expression, in response to salinity, an environmental factor potentially important in determining O. marina spatial distributions.
机译:背景技术在实验,生态学和进化环境下,异养二鞭毛藻滨海种的研究越来越多。其在鞭毛藻中的基础系统发育位置使滨海曲霉对于了解鞭毛虫谱系众多异常特征的起源非常有用。它的广泛分布使O. marina得以研究原生生物地理学;营养柔韧性和造血功能使其成为研究海洋异养鞭毛的生理反应的普通实验动物。然而,用于海洋码头的基因组规模的资源是稀缺的。在这里,我们介绍了这种生物的基于454的转录组调查。另外,我们响应盐度评估了序列读取的丰度,作为基因表达的代理,盐度是一种潜在的环境因子,对确定滨海湾金枪鱼的空间分布具有重要意义。

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