Eliminating Nox2 reactive oxygen species production protects dystrophic skeletal muscle from pathological calcium influx assessed in vivo by manganese‐enhanced magnetic resonance imaging

摘要

Key points class="unordered" style="list-style-type:disc" id="tjp7484-list-0001">Inhibiting Nox2 reactive oxygen species (ROS) production reduced in vivo calcium influx in dystrophic muscle.The lack of Nox2 ROS production protected against decreased in vivo muscle function in dystrophic mice.Manganese‐enhanced magnetic resonance imaging (MEMRI) was able to detect alterations in basal calcium levels in skeletal muscle and differentiate disease status.Administration of Mn²⁺ did not affect muscle function or the health of the animal, and Mn²⁺ was cleared from skeletal muscle rapidly.We conclude that MEMRI may be a viable, non‐invasive technique to monitor molecular alterations in disease progression and evaluate the effectiveness of potential therapies for Duchenne muscular dystrophy.