Fast calcium and voltage-sensitive dye imaging in enteric neurones reveal calcium peaks associated with single action potential discharge

摘要

Non-technical summaryImaging of slow, long-lasting changes in intracellular Ca²⁺ levels ([Ca²⁺]i) is a common method to assess neuronal activity. We found that fast [Ca²⁺]i imaging (≥200 Hz sampling rate) may be a new option to record fast neuronal events including spike discharge and fast synaptic transmission in enteric neurones. These [Ca²⁺]i peaks required opening of voltage-gated sodium and calcium channels as well as Ca²⁺ release from intracellular stores.