首页>
美国卫生研究院文献>The Journal of Physiology
>Supplementation with vitamins C and E inhibits the release of interleukin-6 from contracting human skeletal muscle
【2h】
Supplementation with vitamins C and E inhibits the release of interleukin-6 from contracting human skeletal muscle
Contracting human skeletal muscle is a major contributor to the exercise-induced increase of plasma interleukin-6 (IL-6). Although antioxidants have been shown to attenuate the exercise-induced increase of plasma IL-6, it is unknown whether antioxidants inhibit transcription, translation or translocation of IL-6 within contracting human skeletal muscle. Using a single-blind placebo-controlled design with randomization, young healthy men received an oral supplementation with either a combination of ascorbic acid (500 mg day−1) and RRR-α-tocopherol (400 i.u. day−1) (Treatment, n = 7), or placebo (Control, n = 7). After 28 days of supplementation, the subjects performed 3 h of dynamic two-legged knee-extensor exercise at 50% of their individual maximal power output. Muscle biopsies from vastus lateralis were obtained at rest (0 h), immediately post exercise (3 h) and after 3 h of recovery (6 h). Leg blood flow was measured using Doppler ultrasonography. Plasma IL-6 concentration was measured in blood sampled from the femoral artery and vein. The net release of IL-6 was calculated using Fick's principle. Plasma vitamin C and E concentrations were elevated in Treatment compared to Control. Plasma 8-iso-prostaglandin F2α, a marker of lipid peroxidation, increased in response to exercise in Control, but not in Treatment. In both Control and Treatment, skeletal muscle IL-6 mRNA and protein levels increased between 0 and 3 h. In contrast, the net release of IL-6 from the leg, which increased during exercise with a peak at 3.5 h in Control, was completely blunted during exercise in Treatment. The arterial plasma IL-6 concentration from 3 to 4 h, when the arterial IL-6 levels peaked in both groups, was ∼50% lower in the Treatment group compared to Control (Treatment versus Control: 7.9 pg ml−1, 95% confidence interval (CI) 6.0–10.7 pg ml−1, versus 19.7 pg ml−1, CI 13.8–29.4 pg ml−1, at 3.5 h, P < 0.05 between groups). Moreover, plasma interleukin-1 receptor antagonist (IL-1ra), C-reactive protein and cortisol levels all increased after the exercise in Control, but not in Treatment. In conclusion, our results show that supplementation with vitamins C and E attenuated the systemic IL-6 response to exercise primarily via inhibition of the IL-6 protein release from the contracting skeletal muscle per se.
展开▼
机译:人体骨骼肌收缩是运动引起的血浆白介素6(IL-6)升高的主要因素。尽管抗氧化剂已被证明能减弱运动引起的血浆IL-6的增加,但尚不知道抗氧化剂是否会抑制人骨骼肌收缩中IL-6的转录,翻译或易位。使用具有随机分组的单盲安慰剂对照设计,年轻健康男性接受抗坏血酸(500 mg day -1 sup>)和RRR-α-生育酚(400 iu day)的组合口服补充剂 -1 sup>)(治疗,n = 7)或安慰剂(对照,n = 7)。补充28天后,受试者以其最大最大功率输出的50%进行了3小时动态两腿伸膝运动。在休息(0小时),运动后立即(3小时)和恢复3小时(6小时)后,从外侧臀肌获得肌肉活检。使用多普勒超声检查腿血流量。在从股动脉和静脉采样的血液中测量血浆IL-6浓度。使用菲克原理计算IL-6的净释放。与对照组相比,治疗组血浆维生素C和E浓度升高。血浆8-异前列腺素F2α是脂质过氧化的标志物,在对照组中响应运动而增加,但在治疗中却没有。在对照和治疗中,骨骼肌IL-6 mRNA和蛋白质水平在0至3小时之间均增加。相比之下,腿部的IL-6净释放量(在运动期间增加,在对照组为3.5 h达到峰值)在治疗过程中完全减弱。当两组的动脉IL-6水平达到峰值时,在3至4小时内的动脉血浆IL-6浓度与对照组相比降低了约50%(治疗与对照组:7.9 pg ml -1 sup>,95%置信区间(CI)6.0–10.7 pg ml -1 sup>,而19.7 pg ml -1 sup>,CI 13.8–29.4 pg ml -1 sup>,在3.5小时时,各组之间的P <0.05)。此外,在对照组中,运动后血浆白介素-1受体拮抗剂(IL-1ra),C反应蛋白和皮质醇水平均升高,但在治疗中未升高。总之,我们的结果表明,维生素C和E的补充主要通过抑制从收缩骨骼肌本身释放的IL-6蛋白来减弱运动对全身IL-6的反应。
展开▼