Interstitial fluid pressure (IFP) has been reported to be inversely correlated to rate of perfusion and oxygen tension (pO2) in experimental tumours (Lee et al., 1992; Roh et al., 1991 a). Studies of patients with squamous cell carcinoma of the uterine cervix have provided clinical data consistent with the experimental data (Roh et al., 1991b; Milosevic et al., 1995). These observations have led to the hypothesis that IFP might be a useful indicator of tumour oxygenation status. The purpose of the work reported here was to examine in detail the general validity of this hypothesis. R-18 human melanoma xenografts grown intradermally in Balb/c nuu mice were used as tumour model system. IFP and perfusion rate or IFP and pO2 were measured in the same individual tumours in two independent series of experiments. The wick-in-needle method was used to record IFP. Perfusion rate was studied by using the 86Rb uptake method. The KIMOC-6650 Eppendorf histograph was used to measure pO2. IFP, perfusion rate and pO2 differed considerably between individual tumours. However, there was no relationship between IFP and perfusion rate or IFP and pO2, suggesting that the oxygenation status of tumours cannot be derived from measurements of IFP. Consequently, IFP is probably not a useful predictor of radiation resistance caused by hypoxia.
展开▼
机译:据报道,间质液压力(IFP)与实验性肿瘤的灌注速率和氧张力(pO2)呈负相关(Lee等,1992; Roh等,1991 a)。对子宫颈鳞状细胞癌患者的研究提供了与实验数据一致的临床数据(Roh等,1991b; Milosevic等,1995)。这些发现导致了以下假设:IFP可能是肿瘤氧合状态的有用指标。本文报道的工作目的是详细研究该假设的一般有效性。在Balb / c nu / nu小鼠中皮内生长的R-18人黑素瘤异种移植物用作肿瘤模型系统。在两个独立的实验系列中,在相同的单个肿瘤中测量了IFP和灌注率或IFP和pO2。用芯吸法记录IFP。通过使用86Rb摄取方法研究灌注速率。使用KIMOC-6650 Eppendorf直方图测量pO2。各个肿瘤之间的IFP,灌注率和pO2差异很大。但是,IFP和灌注率或IFP和pO2之间没有关系,这表明不能从IFP的测量中得出肿瘤的氧合状态。因此,IFP可能不是缺氧引起的抗辐射性的有用预测指标。
展开▼