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Cryopreservation for preservation of potato genetic resources

机译:冷冻保存以保护马铃薯遗传资源

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摘要

Cryopreservation is becoming a very important tool for the long-term storage of plant genetic resources and efficient cryopreservation protocols have been developed for a large number of plant species. Practical procedures, developed using in vitro tissue culture, can be a simple and reliable preservation option of potato genetic resources rather than maintaining by vegetative propagation in genebanks due their allogamous nature. Cryopreserved materials insure a long-term backup of field collections against loss of plant germplasm. Occurrence of genetic variation, in tissue culture cells during prolonged subcultures, can be avoided with suitable cryopreservation protocols that provide high regrowth, leading and facilitating a systematic and strategic cryo-banking of plant genetic resources. Cryopreservation protocols for potato reviewed here, can efficiently complement field and in vitro conservation, providing for preservation of genotypes difficult to preserve by other methods, wild types and other species decided as priority collections.
机译:低温保存正成为植物遗传资源长期保存的非常重要的工具,并且已经为许多植物物种开发了有效的低温保存方案。使用体外组织培养技术开发的实用程序可以是马铃薯遗传资源的一种简单而可靠的保存选择,而不是通过其同质性在基因库中通过营养繁殖来维持。冷冻保存的材料可确保长期收集田间作物,以防植物种质流失。可以通过适当的低温保存规程避免组织培养细胞在长时间的继代培养过程中发生遗传变异,该规程可提供高的再生长,从而引导和促进植物遗传资源的系统化和战略性低温储存。此处回顾的马铃薯冷冻保存方案可以有效地补充田间和体外保存,提供难以通过其他方法,野生型和其他物种确定为优先收藏的物种来保存的基因型。

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