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Neospora caninum and complex vertebral malformation as possible causes of bovine fetal mummification

机译:新孢子虫和复杂的椎骨畸形可能是导致牛胎儿木乃伊化的原因

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摘要

Bovine neosporosis, caused by Neospora caninum is a leading cause of abortion in cattle. We postulated that neosporosis could lead to fetal death and mummification. Fifteen mummified fetuses were tested by polymerase chain reaction (PCR) for the mutation in the bovine SLC35A3 gene that causes complex vertebral malformation (CVM) and the pNC-5 gene which identifies N. caninum infection. DNA was extracted from the mummified fetuses and the sex of the mummies was determined by PCR. The CVM mutation was not detected in the mummified fetuses, but 4 fetuses were positive for N. caninum infection. The ages of the mummies with N. caninum infection were 100, 113, 123, and 131 days. Twelve of the 15 mummified fetuses were male. To our knowledge, this is the first detection of N. caninum as a possible cause of bovine fetal mummification.
机译:由犬新孢子虫引起的牛新孢子虫病是牛流产的主要原因。我们推测新孢子虫病可能导致胎儿死亡和木乃伊化。通过聚合酶链反应(PCR)测试了十五只木乃伊胎儿,这些牛中的SLC35A3基因突变引起复杂的椎体畸形(CVM),而pNC-5基因则可以识别犬新孢子虫。从木乃伊的胎儿中提取DNA,并通过PCR确定木乃伊的性别。在木乃伊化的胎儿中未检测到CVM突变,但有4胎对犬新孢子虫感染呈阳性。犬新孢子虫感染的木乃伊的年龄分别为100、113、123和131天。 15个木乃伊胎儿中有12个是男性。据我们所知,这是首次发现犬新孢子虫是牛胎儿木乃伊的可能原因。

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