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Genome-wide Analysis of Alternative Splicing in An Inbred Cabbage (Brassica oleracea L.) Line ‘HO’ in Response to Heat Stress

机译:全基因组分析自交甘蓝(Brassica oleracea L.)品系 HO对热胁迫的选择性剪接

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摘要

Introduction:High-throughput RNA sequencing (RNA-Seq) studies demonstrate that Alter-native Splicing (AS) is a widespread mechanism that enhances transcriptome diversity, particularly in plants exposed to environmental stress. In an attempt to determine the transcriptome and AS patterns of cabbage inbred line “HO” under Heat Stress (HS), RNA-Seq was carried out using HS-treated and con-trol samples. Genome-wide analysis indicated that AS is differentially regulated in response to HS. The number of AS events markedly increased in HS-treated samples compared to the control.
机译:简介:高通量RNA测序(RNA-Seq)研究表明,交替剪接(AS)是增强转录组多样性的广泛机制,尤其是在环境胁迫下的植物中。为了确定在热胁迫(HS)下甘蓝自交系“ HO”的转录组和AS模式,使用经HS处理和控制的样品进行RNA-Seq。全基因组分析表明,AS对HS的反应受到差异调节。与对照相比,经HS处理的样品中AS事件的数量明显增加。

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