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Sequence variation data of the mitochondrial DNA D-loop region of the captive Malayan Gaur (Bos gaurus hubbacki)

机译:被俘马来亚高尔(Bos gaurus hubbacki)的线粒体DNA D环区域的序列变异数据

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摘要

This article contains data of the sequence variation in the mitochondrial DNA D-loop region of the Malayan gaur (Bos gaurus hubbacki), locally known as the seladang, from two captive centers. Thirty fecal samples of Malayan gaur were collected from Jenderak Selatan Wildlife Conservation Center (Pahang) and the Sungkai Wildlife Reserve (Perak) for DNA extraction and amplification with polymerase chain reactions. DNA sequences were then analyzed using neighbor joining (NJ) and maximum parsimony (MP) methods. Based on the 652 base pairs obtained, we found seven variable characters with a value of 1%. The genetic distance between the two captive centers was 0.001. Haplotype analyses detected only four haplotypes between these two captive centers. Both NJ and MP trees demonstrate that all individuals in the Jenderak and Sungkai captive centers are in the same clade. Genetic variation of the Malayan gaur in these centers is considered low, possibly because individuals share the same common parent. This sequence variation data are of paramount importance for designing a proper breeding and management program of the Malayan gaur in the future.
机译:本文包含两个俘虏中心的马来亚人(Bos gaurus hubbacki)的线粒体DNA D环区域中序列变化的数据,该地区在当地被称为seladang。从Jenderak Selatan野生动物保护中心(彭亨)和Sungkai野生动物保护区(霹雳)收集了30份马来亚古尔粪便样本,用于DNA提取和聚合酶链反应扩增。然后使用邻居连接(NJ)和最大简约(MP)方法分析DNA序列。基于获得的652个碱基对,我们发现了七个可变字符,其值均为1%。两个圈养中心之间的遗传距离为0.001。单倍型分析仅检测到这两个俘虏中心之间的四种单倍型。 NJ树和MP树都表明Jenderak和Sungkai俘虏中心的所有个人都在同一个进化枝上。这些中心的马来亚高尔族的遗传变异被认为很低,可能是因为个体拥有相同的共同父母。该序列变异数据对于将来设计适当的马来亚高卢繁殖和管理计划至关重要。

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